lag 3 pe Search Results


lag 3 pe  (R&D Systems)
92
R&D Systems lag 3 pe
Lag 3 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rea351  (Miltenyi Biotec)
95
Miltenyi Biotec rea351
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Rea351, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phycoerythrin pe lag 3  (R&D Systems)
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R&D Systems phycoerythrin pe lag 3
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Phycoerythrin Pe Lag 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd223 antibody  (Miltenyi Biotec)
94
Miltenyi Biotec cd223 antibody
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Cd223 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mabs lag 3  (Miltenyi Biotec)
95
Miltenyi Biotec mabs lag 3
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Mabs Lag 3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse lag3 pe  (Miltenyi Biotec)
93
Miltenyi Biotec anti mouse lag3 pe
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Anti Mouse Lag3 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti lag3 antibodies  (Sino Biological)
92
Sino Biological mouse anti lag3 antibodies
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Mouse Anti Lag3 Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti lag 3 pe  (R&D Systems)
86
R&D Systems anti lag 3 pe
( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + <t>LAG-3</t> + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.
Anti Lag 3 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytokines  (R&D Systems)
90
R&D Systems cytokines
( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + <t>LAG-3</t> + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.
Cytokines, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human lag  (BPS Bioscience)
93
BPS Bioscience anti human lag
( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + <t>LAG-3</t> + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.
Anti Human Lag, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lag 3  (BPS Bioscience)
93
BPS Bioscience lag 3
( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + <t>LAG-3</t> + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.
Lag 3, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd223 antibody, anti-human, reafinity  (Miltenyi Biotec)
95
Miltenyi Biotec cd223 antibody, anti-human, reafinity
( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + <t>LAG-3</t> + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.
Cd223 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Immunophenotyping panel for multiplexed tissue imaging of cancer.

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: LAG3 , REA351 , 25 , 130-120-470 , PE , Miltenyi Biotec.

Techniques: Imaging

( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + LAG-3 + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.

Journal: Scientific Reports

Article Title: Highly-Exposed HIV-1 seronegative Female Commercial Sex Workers sustain in their genital mucosa increased frequencies of tolerogenic myeloid and regulatory T-cells

doi: 10.1038/srep43857

Figure Lengend Snippet: ( a ) Gating strategies by flow-cytometry of an endocervical sample. Cells were first gated on live endocervical cells and then on CD3 + CD4 + T-cells. CD4 + T-cells were selected based on expression of IL-10 + FoxP3 + (Treg) and IL-10 + CD49b + LAG-3 + (Tr1). The frequencies (%) of ( b ) total CD4 + T-cells (left panel) and CD4 + IL-10 − FoxP3 − T-cells (right panel), and ( c ) CD4 + IL-10 + FoxP3 + PD-1 + Treg (left panel) and CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells (right panel) are shown for the three study groups. The geometric mean fluorescence intensity (GeoMFI) of ( d ) PD-1 (right panel), IL-10 (middle panel) and FoxP3 (right panel) is presented for CD4 + IL-10 + FoxP3 + Tregs, and ( e ) PD-1 (right panel), CTLA-4 (middle panel) and LAG-3 (right panel) is presented for CD4 + IL-10 + CD49b + LAG-3 + Tr1 cells. Representative FMO staining controls can be viewed in . Statistical significance of differences in the relative frequencies (%) and levels of expression (GeoMFI) were evaluated with Unpaired T test. *p < 0.05 and **p < 0.001. CSW, commercial sex worker; IL, interleukin; LAP, latency associated peptide; FoxP3, forkhead box P3; PD-1, Programmed cell death protein 1; LAG-3, Lymphocyte activation gene-3.

Article Snippet: The following conjugated mouse or rat anti-human monoclonal antibodies were used: anti-CD3-e fluor 605 NC, anti-CD138-Pe, anti-CD158a-Pe Cy7, anti-CD3/anti-CD19/anti-CD56-Per CP e fluor 710, anti-CD14-e fluor 605 NC, anti-CD11c-Pe, anti-HLA-DR-APC 780, anti-BDCA2-Pe Cy7, and anti-CD11c Pe Cy7, anti-CD103-FITC, anti-CD8-APC 780, anti-LAP-Pe, and anti-FOXP3-488 (eBioscience, San Diego, CA, USA); anti-CD66b-FITC, anti-CD1a-a fluor 700, and anti-CD207-Pe, anti-ILT4-APC, anti-CD56-Brilliant Violet 510, and rat anti-IL-10-Pe Cy7 (BioLegend, San Diego, CA, USA); anti CD19-FITC, anti-CD45-V450, anti-CD209-Pe CF594, anti-HLA-G-biotin/Streptavidin-Pe CF594, anti-IFN-α2b-Pe, anti-TLR7-FITC, anti-CD11c-Pe CF594, HLA-G-Per CP Cy5.5, anti-CD138-BV510, anti-CD4-a fluor 700, and anti-CCR5 (CD 195)-Pe CF504 (BD-Biosciences); anti-Pan Cytokeratin-APC (EXBIO Praha Czech Republic); anti-CD19-Pacific Green (Life Technologies); anti-CD45RA- BV711 (BioLegend, San Diego, CA, USA); anti-LAG-3- Pe, (R&D Systems, Minneapolis, USA); anti-CD3- eVolve 605, anti-ICOS- APC (eBioscience, San Diego, CA, USA); anti-CD45- BUV395, anti-CD49- BV786, anti-CTLA-4- BV421, anti-PD-1- PerCP Cy 5.5, anti-CD8-APC/H7, anti-CD4- a fluor 700, and anti-LAP- PeCF 594 (BD-Biosciences).

Techniques: Flow Cytometry, Expressing, Fluorescence, Staining, Activation Assay